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Blood, Vol. 111, Issue 5, 2733-2743, March 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Activin-A: a novel dendritic cell–derived cytokine that potently attenuates CD40 ligand–specific cytokine and chemokine production Blood Robson et al. 111: 2733 Supplemental materials for: Robson et al Files in this Data Supplement: Figure S1. Activin-A production by other immune cells (JPG, 21.9 KB) - Purified CD4+ or CD8+ T cells or B-cells or NK cells were cultured in 96 well plates for 24h in either culture medium alone or with the indicated stimuli. Supernatants were collected and assayed for activin-A. Grey shaded area indicates the assays level of sensitivity. Data represent the Mean ± 1SD of three separate donors. Figure S2. Direct effect of follistatin on MoDCs IL-6 or IL-12 production and the effects of blocking endogenous activin-A with follistatin on CD40L-mediated IL-6 and IL-12 gene regulation (JPG, 29.3 KB) - Immature MoDCs were cultured in 96 well plates for 24h with either GM-CSF and IL-4 or with follistatin or CD40L (A and D). Supernatants were collected and assayed for IL-6 (A) or IL-12p70 (D) production by ELISA. Data represent the Mean ± 1SD of three separate donors. *p ≤ 0.01 vs follistatin only. MoDCs stimulated with CD40L in the absence or presence of follistatin were lysed, mRNA extracted at times indicated and IL-6 (B) or IL-12p35 (C) qRT-PRC performed. Data shown as one of two separate donors. Figure S3. Effects of activin-A on CD8+ T cell proliferation and IFN-γ production (JPG, 24.5 KB) - Purified CFSE labelled CD8+ T cells were cultured in 96 well plates at 1 × 105/well in medium supplemented with 20 U/ml IL-2 with or without anti-CD3/CD28 bead stimulation in the presence of increasing doses of recombinant activin-A for 24 or 72h. Cell division was assessed over time by flow cytometry and IFN- production measured by ELISA. One representative donor is shown in (A and B) and (C) data represent the Mean ± 1SD of three separate donors. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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