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Blood, Vol. 110, Issue 7, 2696-2703, October 1, 2007
Notch signaling induces cytoplasmic CD3 expression in human differentiating NK cells
Blood De Smedt et al.
110: 2696
Supplemental materials for De Smedt et al, Vol. 110, Issue 7, 2696-2703
Files in this Data Supplement:
- Document 1. Supplemental methods (PDF, 67 KB)
- Figure S1. Kinetics of CD56 expression on sorted CD56−, CD56dim or CD56high cell subsets (JPG, 47.9 KB)
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After 20 days of coculture of CD34+ CB cells on OP9-DL1 cells in the presence of Flt-3L, SCF, IL-7, and IL-15, cells were sorted according to CD56 expression (start) and approximately 20 × 103 to 30 × 103 sorted cells were recultured on OP9-DL1 for different time points, as indicated. Note the appearance and progressive increase in CD56 expression in the sorted CD56− and CD56dim subsets, respectively, whereas the CD56high subset remains CD56high. Histograms are representative of two independent experiments.
- Figure S2. Comparative relative expression levels of CD122 and CD127 mRNA of sorted CD45+CD56+CD3− NK cells (JPG, 28.6 KB)
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Cells were obtained after 22 days of coculture of CD34+ CB cells on OP9-DL1 or OP9-control stromal cells with Flt-3L, SCF; IL-7, and IL-15. The results show mean plus or minus SD of two independent experiments.
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