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Blood, Vol. 110, Issue 12, 4111-4119, December 1, 2007
Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling
Blood Wang et al.
110: 4111
Supplemental materials for: Wang et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 127 KB) -
Contents include detailed protocol for the maintenance of hESCs in DC-HAIF medium and detailed methods for human RTK arrays, receptor inhibition studies, flow cytometry and immunofluorescence, RT-PCR analysis, shRNA vector design and lentiviral infection, cell and colony counting assays in DC-HAIF medium, in vivo and in vitro differentiation of hESCs, Illumina Bead Array and Transcriptional Analysis, equipment and settings and statistical analysis.
- Table S1. Formulation of DC-HAIF medium (XLS, 19 KB)
- Table S2. Expression of ligands, receptors and related downstream signal processing genes in seven hESC lines grown on MEFs or in CM (XLS, 32.5 KB)
- Table S3. Transcriptional analyses of global expression in hESCs maintained in CM and DC-HAIF (Full Dataset) (XLS, 13.2 MB)
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