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Blood, Vol. 111, Issue 2, 829-837, January 15, 2008
ATM kinase activity modulates Fas sensitivity through the regulation of FLIP in lymphoid cells
Blood Stagni et al.
111: 829
Supplemental Materials for: Stagni et al
Files in this Data Supplement:
- Figure S1. A-T cell lines upregulate FLIP and are resistant to Fasinduced apoptosis (PDF, 61 KB) -
(A) 80-100 µg of protein extract prepared from the indicated cell lines were separated by SDS-PAGE, and transferred on nitrocellulose. The proteins of interest and their phosphosphorylation were revealed by immunoblotting with specific antibodies. (B) The indicated cell lines were treated to undergo apoptosis with 500 ng/ml anti-Fas IgM monoclonal antibody. Apoptosis was determined by the analysis of DNA fragmentation in propidium iodide stained cells (P.I).
- Figure S2. Titration of cell lines sensitivity to Fas induced apoptosis (PDF, 202 KB) -
All cell lines were treated to undergo apoptosis with the indicated concentration of anti-Fas IgM monoclonal antibody for the indicated time. Apoptosis was determined by the analysis of DNA fragmentation in propidium iodide stained cells (P.I).
- Figure S3. ATM kinase activity is induced during Fas-induced apoptosis (PDF, 91 KB) -
(A) L6-pcDNA, L6-ATM-wt and L6-ATM-Kin- cell lines were treated to undergo apoptosis with 250 ng/ml anti-Fas IgM monoclonal antibody. Untreated and NCS-treated cells were used as controls. For immunoblotting, 80-100 µg of protein extract were separated by SDS-PAGE, and transferred on nitrocellulose. The proteins of interest and their phosphosphorylation were revealed by immunoblotting with specific antibodies. (B) L6-pcDNA, L6-ATM-wt and L6-ATM-Kin- cell lines were treated to undergo apoptosis with 250 ng/ml anti-Fas IgM monoclonal antibody. Untreated and NCS-treated cells were used as controls. Immunofluorescence microscopic analysis was carried out as previously
Described32. Nuclear condensation and fragmentation has been evaluated by Hoechst staining.
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