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Blood, Vol. 110, Issue 8, 2940-2947, October 15, 2007
Inactivation of PI3K and PI3K distorts T-cell development and causes multiple organ inflammation
Blood Ji et al.
110: 2940
Supplemental materials for Ji et al, Vol. 110, Issue 8, 2940-2947.
Files in this Data Supplement:
- Figure S1. Thymus atrophy in p110γKOδD910A mice (JPG, 85.8 KB).
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(A) Hematoxylin and eosin sections of WT and p110  KO  D910A thymus (Objective lens: ×2). M, Medulla; C, Cortex. Flow cytometry analysis of thymocytes from WT and p110KO D910A mice. (B) CD4 and CD8 profiles gated on living cells. (C) CD25 and CD44 profiles gated on CD4−CD8− cells. (D) Numbers of total, CD4−CD8− (DN), CD4+CD8+ (DP), CD4+CD8− (CD4 SP) and CD4+CD8− (CD8 SP) cells in thymus. Data are from 6 mice in each group (± SEM).
- Figure S2. p110γKOδD910A mice harbor elevated amounts of TNF-α, IFN-γ, MCP-1, and IL-6 (JPG, 94.6 KB).
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Sera were taken from WT and p110KO D910A mice at 7 to 9 weeks of age. Cytometric bead arrays for mouse inflammatory cytokines were applied. Data are from 3 mice in each group (± SEM) and are representative of 6 individual experiments.
- Figure S3. p110γKOδD910A mice do not have circulating autoantibodies to stomach or salivary gland (JPG, 46.5 KB).
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Tissue sections of SCID mice were incubated with serum from WT (1:50 dilution), p110KO D910A (1:50) or MRL-lpr/lpr (1:50) mice and secondary FITC-conjugated  -IgG or Texas Red-conjugated -IgM Ab. (100×/1.30 oil objective or 630/1.40 oil objective). Sections were examined with a Zeiss Axioplan microscope and photographs taken with a Zeiss Axiocam camera (Carl Zeiss, Zurich, Switzerland) and Nikon ACT-1 software (version 2.12).
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