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Blood, Vol. 110, Issue 10, 3673-3681, November 15, 2007
Treatment with GITR agonistic antibody corrects adaptive immune dysfunction in sepsis
Blood Scumpia et al.
110: 3673
Supplemental materials for: Scumpia et al
Files in this Data Supplement:
- Figure S1. Anti-GITR treatment increases T cell dependent antibody responses in mice (PDF, 16.3 KB) -
C57Bl/6 mice were given an intraperitoneal injection of 300 µg control antibody or anti-GITR antibody and were immunized with NP-KLH in alum 30 minutes later. 10 days later mice were bled and anti-NP specific IgM (A), IgG2a (B), and IgG1 (C) were measured from the serum.
- Figure S2. Sepsis alters the cell surface phenotype of T cells (PDF, 43.4 KB) -
24 hours after cecal ligation and puncture (CLP) or sham surgery, splenocytes were harvested and flow cytometry was performed. Left panels show representative examples examining A) CD28, B) CTLA-4, C) CD69 and D) GITR expression on living (Sytox Blue−) CD3+CD4+ T cells from sham (black histogram) or CLP (grey histogram). Right panels show the mean fluorescent intensity (MFI) of CD28, CTLA-4, or GITR expression, or the percentage of CD69+ TH cells from sham (n=3) or CLP (n=4) mice. * p < 0.05 by Student’s t test.
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