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Blood, Vol. 111, Issue 4, 2374-2377, February 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef DNA methylation-independent loss of RARA gene expression in acute myeloid leukemia Blood Glasow et al. 111: 2374 Supplemental materials for: Glasow et al Files in this Data Supplement: Figure S1. PRAME expression levels were calculated by real-time RT-PCR as in Figure 1A (JPG, 43.3 KB) - The samples analyzed were as follows: normal cord blood (CB) or peripheral blood (PB) cells (n=4, consisting of CD33 PB, CD33 CB, CD34 CB and CD133 CB); primary non-APL AML cells from consenting patients (n=7, consisting of AML FAB subtypes M1, M2, M4 and M5); primary APL cells (AML M3) from consenting patients (N=8); AML cell lines (n=3; Kasumi-1, THP1 and U937). “n” represents the number of individuals or cells lines. K562 cells were used as a positive control. The following PCR primers were used: PRAME-Fwd 5′-GCAGGCTCTCTCTATGTGGACTCTT-3′; PRAME-Rev 5′-GACCCCACTTAGACTCAGGACAC-3′. Figure S2. RARβ2 expression levels were calculated by real-time RT-PCR as in Figure 1B except indicated normal cord blood cells and AML patient samples (n=10) are shown relative to the value obtained for AML M1 patient P2 (JPG, 44 KB) - The following PCR primers were used: RAR2-Fwd 5′-CTTCCTGCATGCTCCAGGA-3′; RAR2-Rev 5′-CGCTGACCCCATAGTGGTA-3′. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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