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Blood, Vol. 111, Issue 1, 150-159, January 1, 2008
M-CSF: a novel plasmacytoid and conventional dendritic cell poietin
Blood Fancke et al.
111: 150
Supplemental material for: Fancke et al
Files in this Data Supplement:
- Figure S1. Surface phenotype of M-cDC compared to FL-cDC and ex-vivo isolated spleen cDC (PDF, 102 KB) -
Stained cells from day 6 M-CSF cultures (filled histograms), FL cultures (grey open histograms) or freshly isolated spleen DC (black histograms) were gated on cDC by selecting for the expression of CD11c and lack of CD45RA or CD45R amongst the PI negative cells. The expression of a range of surface markers on the cDC surface is shown. The light grey histograms represent the background staining of the M-cDC within each stain. All M-cDC also lacked expression of CD3, CD19, CD49b and NK1.1. The surface phenotypes shown are from one experiment representative of 2-5 experiments for M-cDC, 2-3 experiments for FL-cDC and 2 experiments for spleen cDC.
- S2. The generation of M-cDC and M-pDC is inhibited by a c-FMS inhibitor and the generation of FL-cDC and FL-pDC is inhibited by a Flt3 inhibitor (PDF, 56 KB) -
The replicate FL (A) and M-CSF (B) cultures of Figure 4 conducted in the presence of inhibitors were also analysed by FACS for pDC and cDC subpopulations. The number of cells within pDC and cDC subpopulations harvested from cultures without inhibitors was set at 100%. Cells from cultures containing inhibitor were expressed as a percentage of cells obtained in the absence of inhibitor.
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