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Blood, Vol. 111, Issue 10, 5109-5117, May 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Transgenic expression of JAK2V617F causes myeloproliferative disorders in mice Blood Xing et al. 111: 5109 Supplemental materials for: Xing et al Files in this Data Supplement: Figure S1. Real time PCR analyses of JAK2V617F expression in transgenic mice (JPG, 55.7 KB) - Real time PCR assays were performed with 0.03, 0.3, 3, 30 pg purified standard mouse Jak2 or human JAK2 plasmid DNAs diluted in 20 µg/ml salmon sperm DNA as a carrier. Genomic DNAs from mouse tails were analyzed in parallel. Data (PCR threshold cycles vs. DNA dilutions) show results obtained with 30 ng genomic DNAs from the founder mice A (×), B (+), and C (). Figure S2. Tissue distributions of endogenous mouse Jak2 expression in transgenic mice (JPG, 39.3 KB) - Expression levels of endogenous mouse Jak2 in the indicated tissues of line A transgenic mice were analyzed with real time PCR in conjunction with assays of human JAK2V617F (see Fig. 1). Data represent relative mRNA levels (mean ± SD, n ≥ 3) normalized to mouse GAPDH in arbitrary units. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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