|
|
Blood, Vol. 111, Issue 7, 3489-3497, April 1, 2008
Basal and angiopoietin-1–mediated endothelial permeability is regulated by sphingosine kinase-1
Blood Li et al.
111: 3489
Supplemental materials for: Li et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 15.3 KB)
- Figure S1. The VEGF levels in serum from WT, SK−/− and SK+/+ mice (six per group) were measured (JPG, 37.8 KB)
-
Data is presented as means ± SD. NS, no significant difference compared to WT.
- Figure S2. mRNA levels were determined 48 h after transfection with control siRNA, S1P1 siRNA (A) or control siRNA, S1P3 siRNA (B) (JPG, 53.9 KB)
-
Data are presented as percentage change in comparison with control siRNA transfected cells. *p < 0.001 compared to control siRNA cells.
- Figure S3 (JPG, 61.2 KB)
-
(A) Confocal micrographs of HUVE cells transfected with hSK-1-GFP: i without treatment, ii treated with Ang-1 (0.2 µg/ml) for 30 min. (B) Immunoblot of cytosol and membrane fractions of hSK1–FLAG-transfected HUVE cells, Untreated cells (–) or cells treated with Ang-1 for 30 min (+). The membrane associated protein, VE cadherin, was used as the fractionation and loading control.
- Figure S4 (JPG, 84.4 KB)
-
(A) HUVE cells infected with adenovirus as either (i) empty vector or (ii) carrying the hSK-1 cDNA were replated onto LabTek slides 48 h after infection. The cells were washed and fixed 4 h after plating. Cells were stained with rhodamine phalloidin. (B) HUVE cells were infected with adenovirus carrying either empty vector (EV) or the hSK-1 gene (SK-1). After 48 h, cells were lysed and Rac activity measured. Active Rac is shown in the upper panel and total Rac is shown in the lower panel. (C) HUVE cells were infected with adenovirus carrying either empty vector (EV) or the hSK-1 gene (SK-1). After 48 h, cells were lysed and Rho activity measured. Active Rho is shown in the upper panel and total Rho is shown in the lower panel.
|
|
