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Blood, Vol. 112, Issue 6, 2563-2574, September 15, 2008
In vivo trafficking and survival of cytokine-induced killer cells resulting in minimal GVHD with retention of antitumor activity
Blood Nishimura et al.
112: 2563
Supplemental materials for: Nishimura et al
Files in this Data Supplement:
- Figure S1. No impact of exogenous IL-2 administration on CIK kinetics in vivo (JPG, 73.6 KB)
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Irradiated host Balb/c mice were injected with 5 × 106 BM cells + 10 × 106 CIK cells generated from C57Bl6 (luc+) with or without exogenous administration of IL-2 (50,000U/mouse/day). (A) BLI images on day 2 and 8. (B) Quantitative analysis of BLI over time. (C) Survival curve. (D) Proliferation index of donor derived CD4 and CD8 T cells from CIK cell cultures recovered from the spleen on day 3 after BMT. (E) Percentage of apoptotic cells derived from injected CD4 and CD8 T cells from CIK cell cultures recovered from the spleen on day 7 after BMT. Results are derived from 3 independent experiments.
- Figure S2. NKG2D ligands are not up-regulated on GVHD target tissues after irradiation (JPG, 77.5 KB)
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NKG2D ligand (MULT1) was expressed on A20 tumor cells, but not on GVHD target tissues such as small and large bowel, skin, and liver on day 3 and 5 after irradiation.
- Figure S3. GVT effects of CIK cells in a dose-dependent manner without GVHD (JPG, 141 KB)
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1 × 106 A20 luc+ cells were injected into the right flank of Balb/c hosts subcutaneously after lethal irradiation, followed by injection of 5 × 106 C57BL/6 wild type TCD-BM with the indicated doses of CIK cells and splenocytes generated from congenic C57BL/6 animals. CIK high, 20 × 106; CIK medium, 10 × 106; CIK low, 5 × 106; Splenocytes high, 10 × 106; Splenocytes medium, 5 × 106; Splenocytes low, 2.5 × 106. (A) GVT effects of different CIK cell doses plotted with (left) or without (right) tumor only control. (B) Percent body weight change over time. The animals treated with CIK cells showed much less GVHD as compared with animals receiving even low dose of splenocytes.
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