|
|
Blood, Vol. 110, Issue 9, 3398-3406, November 1, 2007
The Laminin 511/521–binding site on the Lutheran blood group glycoprotein is located at the flexible junction of Ig domains 2 and 3
Blood Mankelow et al.
110: 3398
Supplemental materials for: Mankelow et al
Files in this Data Supplement:
- Document 1. Small-Angle X-ray scattering analysis — full method (PDF, 73.5 KB)
- Figure S1. Sequence of Lu gp D1D2 (JPG, 36.3 KB)
-
The  -strands observed in the crystal structure are indicated by shaded boxes and labeled with bold letters. Mutated residues that affect binding are indicated with boxes and labeled with asterisks.
- Figure S2. Western blots using Bric 224 (JPG, 19.1 KB)
-
(A) Followed by a rabbit anti-mouse HRP secondary Ab was performed on non-reduced samples (0.2 µgs) and using a anti human Fc HRP conjugate. Lanes are i-E132A/D133A, ii-E180A, iiiD198A/D199A, iv E234A, v-D269A, vi-D280A, vii-D281A, viii- E309A, ix- E310A, x- D312A, xi- D315A, xii- D316A and xiii- Native Lu. Non- reduced Western blots using Bric 224 (B) of  233-235 (i), T233P (ii) H235P (iii), linker mutations and native Lu (iv) are shown.
- Figure S3. Representative ELISA titrations of 5, 0.5, 0.05 and 0.005nM Lu gp binding to 5nM Ln511/521 for the Mutated Lu gpFc (Blue) mutations in comparison to native Lu gp (Magenta) (JPG, 146 KB)
-
Standard deviation for each point is under 0.1.
|
|
