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Blood, Vol. 111, Issue 6, 3257-3265, March 15, 2008
Indoleamine 2,3-dioxygenase is a critical regulator of acute graft-versus-host disease lethality
Blood Jasperson et al.
111: 3257
Supplemental material for: Jasperson et al
Files in this Data Supplement:
- Figure S1. GVHD does not upregulate IDO in liver or skin (PDF, 44 KB) -
B6 or IDO-/- mice were lethally irradiated and infused with Balb/c TCD BM with or without 3 million CD4+ and CD8+ T cells. Seven days later, tissues were harvested, fixed, paraffin embedded, and stained by IHC for IDO. Pictures are representative of 3-5 mice/group.
- Figure S2. IDO is increased in GVHD spleens (PDF, 152 KB) -
B6 mice were lethally irradiated and infused with Balb/c TCD BM with or without 3 million CD4+ and CD8+ T cells. Seven days later, spleens were harvested, fixed, paraffin embedded, and stained by IHC for IDO. Pictures are representative of 3-5 mice/group.
- Figure S3. GVHD histopathology is equivalent in small intestine, liver, and skin (PDF, 40 KB) -
B6 or IDO-/- mice were lethally irradiated and infused with Balb/c TCD BM with or without 3 million CD4+ and CD8+ T cells. 27 days later, tissues were harvested and cryosections were stained with H&E. Pictures are representative of 3-5 mice/group.
- Figure S4. Host IDO deficiency does not increase radiation sensitivity (PDF, 21 KB) -
A.B6 or IDO-/- mice were sublethally irradiated (5.0 Gy), and weights were monitored twice weekly. Weights are shown as percent of weight on day -1. No mice died. n=4 mice/group. B.Mice were lethally irradiated (8.0 Gy) and infused with 10x106 B6 (syngeneic) TCD or NTCD BM, and weights were monitored twice weekly. Weights are shown as percent of weight on day -1. No mice died. ●, B6 NTCD BM→B6; ■, B6 TCD BMοB6; ○, B6 NTCD BM→IDO-/-; □, B6 TCD BMοIDO-/-; n=5 mice/group.
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