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Blood, Vol. 110, Issue 9, 3271-3280, November 1, 2007
PPAR regulates the function of human dendritic cells primarily by altering lipid metabolism
Blood Szatmari et al.
110: 3271
Supplemental materials for: Szatmari et al
Files in this Data Supplement:
- Figure S1. Results of the comparison the number of the transcripts regulated by PPARγ ligand treatment or human serum (PDF, 110 KB) -
Monocytes were cultured for 6, 24 hours or 5 days as described in Materials and Methods. The indicated cells were cultured in human AB serum (DC HS) instead of FBS. Cells were treated with 1 µM rosiglitazone (RSG; 6 hours, 24 hours DC) or with 2.5 µM RSG (5 days DC). The number of the probe sets which’s expression was at least 2 fold (upregulated genes) or less than 0.5 (downregulated genes) comparing RSG versus FBS cultured cells, or HS versus FBS cultured cells, with p values less than 0.01. Microarray data are based on 3 biological replicates.
- Table S1. PPARγ regulated transcripts (XLS, 222 KB)
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This table shows the 6 genelists containing all of the Affymetrix probe sets which were up (RSGup) or downregulated (RSGdown) comparing vehicle versus PPAR ligand (rosiglitazone abbreviated as RSG) treated cells. Cells were harvested after 6, 24 hours or 5 days (6h, 24h, 5d) and they were treated with 1 µM RSG (6h, 24h) or with 2.5 µM RSG (5d). Probe sets were selected as described in Materials and Methods. Fold change by RSG is shown relative to vehicle treated cells. Microarray data were obtained utilizing 6 biological replicates.
- Table S2. Overrepresented Gene Ontology (GO) categories on PPARγ regulated transcripts (XLS, 29.5 KB)
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Microarray gene lists (Table S1) were analyzed with Cytoscape/BiNGO software to assess GO category enrichment. This table shows 6 lists containing all of overrepresented GO-Biological process categories. The corrected p values were calculated by Cytoscape/BiNGO software using hypergeometrical test and Benjamini and Hochberg False Discovery Rate (FDR) correction.
- Table S3. Validated PPARγ regulated transcripts (XLS, 29 KB)
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This table shows the 4 genelists containing all of the transcripts which were up (RSGup) or downregulated (RSGdown) comparing vehicle versus PPAR ligand (rosiglitazone abbreviated as RSG) treated cells using Taqman low density array (TLDA) analyses. Cells were harvested after 6, 24 hours (6h, 24h) and they were treated with 1 µM RSG. Genes were selected if the fold change was at least 1.5 and p value was less than 0.05. Fold change by RSG is shown relative to vehicle treated cells. Real-time quantitative RT-PCR data were obtained utilizing 3 biological replicates.
- Table S4. Human serum regulated transcripts (XLS, 523 KB)
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This table shows the 6 genelists containing all of the probe sets which were up (HSup) or downregulated (HSdown) comparing FBS versus human serum (HS) cultured cells. Cells were harvested after 6, 24 hours or 5 days (6h, 24h, 5d). Probe sets were selected as described in Materials and Methods. Fold change by human serum is shown relative to FBS cultured cells. Microarray data were obtained utilizing 3 biological replicates.
- Table S5. Real-time quantitative RT-PCR (qRT-PCR) primers and probes (XLS, 29 KB)
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