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Blood, Vol. 111, Issue 1, 271-274, January 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Lack of iNKT cells in patients with combined immune deficiency due to hypomorphic RAG mutations Blood Matangkasombut et al. 111: 271 Supplemental materials for: Matangkasombut et al Files in this Data Supplement: Figure S1. Flow cytometric analysis of peripheral blood mononuclear cells (PBMC) from 6 healthy controls (JPG, 58.4 KB) - Leukocytes were enumerated by gating on CD45+CD3+ cells. iNKT cells were identified using PBS57-loaded CD1d tetramers (right panel) using cells stained with unloaded CD1d tetramers as control (left panel). In the 6 controls, iNKT cells represent between 0.1 to 0.7 % of CD3+ cells (from 547 to 6265 iNKT cells per ml of blood). Figure S2. Flow cytometric analysis of peripheral blood mononuclear cells (PBMC) from patient 3 and 5 and a healthy control using three different methods to identify iNKT cells (JPG, 93.5 KB) - Leukocytes were enumerated by gating on CD45+CD3+ cells. iNKT cells were identified using a) PBS57-loaded CD1d tetramers (top left) using cells stained with unloaded CD1d tetramers as control (bottom left) b) anti-invariant NKT cells antibody (clone 6B11) (top middle) and isotype antibody control (bottom middle) and c) anti V24/V11 antibody (top right) and isotype antibody control (bottom right). This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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