|
|
Blood, Vol. 110, Issue 13, 4278-4284, December 15, 2007
Impaired T-cell priming in vivo resulting from dysfunction of WASp-deficient dendritic cells
Blood Bouma et al.
110: 4278
Supplemental materials for: Bouma et al
Files in this Data Supplement:
- Figure S1. Similar maturation phenotype of C57BL/6 and WAS KO DC (JPG, 242 KB)
-
Surface expression of CD80, CD86, CCR7, and MHC class II molecules was analysed of WAS KO and C57BL/6 DC before injection. No differences were observed between C57BL/6 and WAS KO DC.
- Video 1. Migration of C57BL/6 DC (WMV, 607 KB)
-
Movie of individual immature C57BL/6 DC migrating in a Dunn chamber in response to CCL3, showing normal leading edge formation, spreading of the cell body and net forward motion. Movie was recorded by taking images at 10 min intervals over a 5 hour period.
- Video 2. Migration of WAS KO DC (WMV, 599 KB)
-
Movie of individual immature WAS KO DC migrating in a Dunn chamber in response to CCL3. WAS KO DC failed to form a persistent leading edge and did not spread their cell body, resulting in a strongly reduced translocation and seemingly random movement around their point of origin without successful displacement. Movie was recorded by taking images at 10 min intervals over a 5 hour period.
- Video 3. 3D reconstruction of migrated C57BL/6 DC (WMV, 5.61 MB)
-
3D reconstruction of CFSE+ C57BL/6 DC that migrated to the draining lymph node 24hrs after injection. DC are pseudocoloured in red. Total thickness of lymph node that was imaged was 50 µm. The curving of the subcapsular area can be appreciated at the start of the movie on the left-hand side. Several cells can be observed to be present deep inside the lymph node.
- Video 4. 3D reconstruction of migrated WAS KO DC (WMV, 5.65 MB)
-
3D reconstruction of CFSE+ WAS KO DC that migrated to the draining lymph node 24hrs after injection. DC are pseudocoloured in red. Total thickness of lymph node that was imaged was 50 µm. The curving of the subcapsular area can be appreciated at the start of the movie on the left-hand side. Most cells seem stuck in the subcapsular area. Note that due to high autofluorescence of subcapsular macrophages not all highlighted cells will be CFSE+ DC, but it can be appreciated that very few, if any, cells managed to penetrate deeper into the lymph node.
|
|
