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Blood, Vol. 111, Issue 10, 5028-5036, May 15, 2008
Platelet-mediated modulation of adaptive immunity: unique delivery of CD154 signal by platelet-derived membrane vesicles
Blood Sprague et al.
111: 5028
Supplemental materials for: Sprague et al
Files in this Data Supplement:
- Figure S1. Centrifugation to isolate CD154 containing fraction of activated platelet supernatant (JPG, 66.3 KB)
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Isolated mouse platelets were activated for 20 minutes at 37°C then centrifuged for 5 minutes at 13,000 xg. The resultant supernatant was then centrifuged at 10,000xg, 20,000xg, 50,000xg, or 100,000xg for 2 hours at 4°C. Then PDMV pellet (PDMV) and PDMV-poor supernatant (PDMV-poor Sup) were injected iv into CD154 knock out mice 24 hours after immunization with Ad-OVA. Serum was collected on day 7 for quantification of Adenovirus-specific antibody production. Results are shown as optical absorbance at 490nm.
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