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Blood, Vol. 111, Issue 4, 2073-2082, February 15, 2008
In vivo transformation of mouse conventional CD8 + dendritic cells leads to progressive multisystem histiocytosis
Blood Steiner et al.
111: 2073
Supplemental materials for: Steiner et al
Files in this Data Supplement:
- Figure S1. Loss of normal structure in affected organs (JPG, 78.2 KB)
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Histological analysis of DC in littermate (LM) and diseased Mushi1 mice (TG) spleen, liver and peripheral LN (per. LN) stained by hematoxilin&eosin.
- Figure S2. Extensive infiltration of lymphoid organs by CD11c+GFP+ cells (JPG, 174 KB)
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FACS analysis of DC content of spleen, bone marrow (BM), peripheral LN (pLN), mesenteric lymph node (MLN), thymus and PBL of healthy WT and diseased Mushi1 (TG sick) mice. The percentages of WT DC (CD11c+ GFP−) or Tg DC (CD11c+ GFP+) are indicated.
- Figure S3. Pathological cells are CD8α+ cDCs expressing CD24 and CD205 (JPG, 106 KB)
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FACS analysis of DC content of spleen of 3.5 month-old littermate (LM) and Mushi1 (TG) mice. In the left panels, the % of CD11c+ and CD11c− among live-gated cells are shown. In the right panels, the % of CD11c+-gated cells expressing CD8 and CD205 are shown.
- Figure S4. Expression profile of pathological cells for IRF-4, IRF-8, CIITA-I, Langerin, DC-Sign and CD68 is closest to CD8α+ cDCs (JPG, 75.7 KB)
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The indicated mRNAs were quantified by real-time RT-PCR in FACS purified epidermal LCs, spleen CD8− and CD8+ cDCs from indicated mice using an absolute quantification titration curve.
- Figure S5. Tg expression is highest in CD8α+ cDCs (JPG, 38 KB)
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Tg mRNA levels were quantified by real-time RT-PCR in brain and FACS purified spleen CD8− and CD8+ cDCs and epidermal LCs from 3.5 month old Mushi1 mice. RNA isolated from Tg brain was used to show background level.
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