Blood, Vol. 111, Issue 8, 4309-4321, April 15, 2008
Mutation of C/EBP predisposes to the development of myeloid leukemia in a retroviral insertional mutagenesis screen Blood Hasemann et al. 111: 4309 Supplemental materials for: Hasemann et al Files in this Data Supplement: Document 1. Supplemental materials and methods (PDF, 75.1 KB) Table S1. Sequence of 182 SRS19-6 retroviral insertion sites (RIS) in 67 tumors from 3 genotypes (XLS, 46 KB) Figure S1. Characterization of the double-negative (DN) thymocytes in CebpaBRM2/BRM2 mice (JPG, 158 KB) - (A) FACS analysis of DN thymocytes from Cebpa+/+ and CebpaBRM2/BRM2 mice revealed no differences between these animals in the frequencies of DN1 (CD25−CD44−), DN2 (CD25−,CD44+), DN3 (CD25+,CD44+) and DN4(CD25+,CD44−). (B) Quantification of the results in (A). 4 mice of each genotype were used. Figure S2. Gene-specific southern blot analysis of mice bearing retroviral integrations (JPG, 177 KB) - Blow-up of the Southern blots from Figure 7. Spleenic genomic DNA was isolated from WT (N) and diseased (T) animals and subjected to southern blot analysis using gene-specific probes. Bands representing retroviral integration sites are indicated by asterisk (left of the band).
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