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Blood, Vol. 110, Issue 13, 4503-4510, December 15, 2007
Tissue-specific histone modification and transcription factor binding in  globin gene expression
Blood De Gobbi et al.
110: 4503
Supplemental materials for: De Gobbi et al
Files in this Data Supplement:
- Figure S1. Histone H4 and H3 acetylation at the α globin locus during terminal erythropoiesis (JPG, 103 KB)
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Real-time q-PCR analysis of ChIP performed with antibodies against H4ac (A), H3ac (B) is shown. For each panel a schematic representation of the  globin locus is shown at the top, including DHSs, MCS-R and real time PCR amplicons. The y axis represent average enrichment over input DNA from two independent ChIPs, normalized to a control sequence in the 18S ribosomal RNA gene and compared to the value obtained for human  actin promoter. The x axis is in scale with the globin locus. The results from the different stages of erythrobast maturation, as in Figure 2, are shown. Results from primary T lymphocyte (T Ly) and K562 are also shown. Elements corresponding to the highest peaks of enrichment are labeled. Asterisks indicate where qPCR analysis was not performed.
- Figure S2. Transcription factor and PolII binding at α globin locus during terminal erythroid differentiation (JPG, 201 KB)
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Real-time q-PCR analysis of ChIP performed with antibodies against GATA1 (A), SCL (B), NF-E2 p45 (C), NF-E2 p18 (D) and Pol2 (E). The data are plotted as for Figure S1. Elements corresponding to the highest peaks of enrichment are labeled. Asterisks indicate where qPCR analysis was not performed.
- Figure S3. GATA1-SCL pentameric complex at the α globin locus in K562 cell line (JPG, 85.6 KB)
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Real-time q-PCR analysis of ChIP performed with antibodies against GATA1, SCL, Ldb1, LMO2 and E2A. The data are plotted as for Figure S1. Elements corresponding to the highest peaks of enrichment are labeled.
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