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Blood, Vol. 111, Issue 7, 3859-3862, April 1, 2008
Npm1 is a haploinsufficient suppressor of myeloid and lymphoid malignancies in the mouse
Blood Sportoletti et al.
111: 3859
Supplemental materials for: Sportoletti et al
Files in this Data Supplement:
- Figure S1. Additional features of Npm1+/− mice hematological malignancies (JPG, 822 KB)
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(A) Npm1+/− enlarged spleen is compared to a wild-type littermate control. (B) 10× (left panel, arrows) and 40× (right panel) magnification of perisinusoidal infiltrates in a representative Npm1+/− mouse liver composed of atypical mononuclear cells. (C) 10× (upper panel, arrow heads) and 40× (lower panel) magnification of a perirenal atypical mononuclear infiltrate. (D) Peri-intestinal tumor mass composed of atypical mononuclear infiltrate (upper panel 10×, lower panel 40× magnification). (E) 10× magnification of atypical lymphoid infiltrate replacing splenic architecture. The infiltrate is positive for CD20 (inset, 40× magnification) supporting involvement by a B-cell lymphoma. (F) Liver parenchyma (10× magnification) partially replaced by an atypical lymphoid infiltrate (arrow heads). (G) 10× magnification of atypical lymphoid infiltrate of spleen. The infiltrate is positive for CD3 (inset, 40× magnification) supporting involvement by a T-cell lymphoma.
- Figure S2. Npm1 expression levels and genomic instability in Npm1+/− mice hematological malignancies (JPG, 437 KB)
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(A) Hematoxylin and eosin (H&E) staining (upper panels) and immunohistochemical analysis of Npm1 expression (lower panels) in spleen sections from ML affected mice (middle and right panels) compared to a Npm1+/+ mouse as control (left panels). The insets show normal nuclear localization of Npm1. (B) Centrosome amplification in MPD-like ML cells. Representative bone marrow cytospins from Npm1+/− mouse without tumors and Npm1+/− mouse affected with MPD-like ML stained with an anti- -tubulin (red) and DAPI (blue) are shown. White arrows point centrosomes. (C) Chromosomal abnormalities detected using CGH array analysis on haematological malignancies of six Npm1+/− mice. Chromosome gains and losses are listed as numerical abnormalities. The presence of structural abnormalities, such as deletion and duplication of chromosome regions, is indicated for each mouse.
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