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Blood, Vol. 111, Issue 6, 3024-3033, March 15, 2008
RhoA activation and actin reorganization involved in endothelial CAM-mediated endocytosis of anti-PECAM carriers: critical role for tyrosine 686 in the cytoplasmic tail of PECAM-1
Blood Garnacho et al.
111: 3024
Supplemental materials for: Garnacho et al
Files in this Data Supplement:
- Figure S1. Anti-PECAM antibody Ab62 and anti-PECAM/NCs bind specifically to PECAM-1 (JPG, 83.9 KB)
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(A) HUVEC (i), RhP (ii) and REN (iii) cells were fixed in cold 2% paraformaldehyde and immunostained with anti-PECAM antibody Ab62. (B) HUVEC (i), RhP (ii) and REN (iii) cells were incubated with FITC-labeled anti-PECAM/NCs at 37°C for 1 hour, washed, fixed, and counterstained with Texas red goat anti-mouse IgG to label non-internalized nanocarriers, as in Figure 4. Insets in A(iii) and B(iii) show the corresponding phase-contrast image. Magnification bar = 10 µm.
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