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Blood, Vol. 111, Issue 1, 160-164, January 1, 2008
Simultaneous loss of β- and  -catenin does not perturb hematopoiesis or lymphopoiesis
Blood Koch et al.
111: 160
Supplemental materials for: Koch et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 75.6 KB)
- Figure S1. Western blot analysis on isolated wild type BM cells, thymocytes and splenocytes showing the expression of β-catenin (first lane), γ-catenin (second lane) and tubulin (third lane) used as a loading control (JPG, 45.9 KB)
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One hundred µg of protein were loaded for each sample.
- Figure S2. Representative FACS analysis showing the stem cell compartment of control and β/γ dko FL chimeras stained with anti-CD150 and anti-CD34 antibodies, both gated on CD45.2+ KLS BM donor cells (JPG, 82.1 KB)
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- Figure S3. Bar diagrams represent absolute numbers ± SD of either CD45.2+ control or β/γ dko BM cells from FL chimeras (JPG, 52.2 KB)
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(A) Myeloid lineages including granulocytes, Gr (Gr1+/Mac1) macrophages, MØ (Mac1+Gr1−), megakaryocytes, Mega (CD41+), early erythroblasts, EB (Ter119+CD71+) and red blood cells, RBC (Ter119+CD71−). (B) Different BM B cell stages including pro/preB cells (B220+CD43+BP1−), large pre B II cells (preBII) (B220+CD43+BP1+) small preB II cells (spreBII) (B220+CD43−BP1+IgM−) and immature B cells (Imm) (B220+CD43−BP1−IgM+) gated on CD45.2+ cells (n= 7 for control and 8 for  / dko). Student’s t-test was performed on all populations indicated and showed no significant differences (p-value > 0,3).
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