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Blood, Vol. 111, Issue 3, 1437-1447, February 1, 2008
A common pathway mediated through Toll-like receptors leads to T- and natural killer–cell immunosuppression
Blood Vaknin et al.
111: 1437
Supplemental materials for: Vaknin et al
Files in this Data Supplement:
- Figure S1. ζ-chain down-regulation and impaired T cell function following BCG treatment (JPG, 107 KB)
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C3H/HeN mice were repeatedly exposed to heat-killed BCG was administered by 3 subcutaneous injections at 1wk intervals, 70 µg per animal/dose; the first two doses were administrated with IFA, and subsequent injections included heat-killed BCG in PBS. (A) At day 1, splenic Thy1.2+ T cells from BCG-treated and control mice were analyzed for total  and CD3e expression levels by FACS. (B) Splenocytes from BCG-treated and control mice were activated as described in Materials and Methods. Specific T cell proliferation was measured by intracellular staining for BrdU gated on Thy1.2+ T cells. The results are presented as the mean value of three independent experiments, and standard deviations are shown. *, P < 0.001 (Student’s t-test). (C) BCG-treated and control C3H/HeN mice were subjected to adoptive transfer with CFSE-labeled naïve splenocytes at day -2. At day 1, -chain expression was measured in CFSE+Thy1.2+ cells from control and BCG-treated mice (left panel). Anti-CD3 antibodies were then injected i.p. and on day 3 splenic cells were harvested and the divisions of CFSE+Thy1.2+ cells were analyzed by FACS (right panel). The upper two dot blots represent non activated splenic T cells (CFSE+Thy 1.2+ cells) from control or BCG-treated mice, while the lower two dot-blots represent anti-CD3 activated CFSE-labeled T cells from control or BCG-treated mice. A representative experiment is shown out of three performed.
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