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Blood, Vol. 111, Issue 3, 1428-1436, February 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities Blood Diermayr et al. 111: 1428 Supplemental materials for: Diermayr et al Files in this Data Supplement: Table S1. Ligands on myeloblastic/monoblastic cells and in plasma of AML (n=66) and CMML(n=3) patients at diagnosis (XLS, 38.5 KB) Table S2. HLA-class I haplotype of patients and potential NK cell allorecognition by KIR-HLA-class I mismatch (XLS, 16.5 KB) Figure S1 (JPG, 29.6 KB) - (A) A biochemical ULBP1 detection approach in which human plasma was subjected to fractionation by size exclusion chromatography followed by ELISA analysis of the eluate (see Methods). ULBP1 eluted as a single peak with an estimated molecular weight of 63 kDa. (B) The ULBP1-containing fractions (21-25) were pooled and subjected to immunoprecipitation and Western blot analysis. ULBP1-specific mAbs immunoprecipitated a protein of about 20 kDa (lane 4), as predicted for the extracellular ULBP1 region, which was not observed in control immunoprecipitations using mouse IgG1 (lane 2) or NKG2D receptor-specific mAbs (lane 3). Rh ULBP1/Fc is loaded as a control (lane 1). This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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