|
|
Blood, Vol. 111, Issue 5, 2714-2724, March 1, 2008
SWAP-70 deficiency causes high-affinity plasma cell generation despite impaired germinal center formation
Blood Quemeneur et al.
111: 2714
Supplemental materials for: Quemeneur et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 59.9 KB)
- Figure S1. GC formation reduction in SWAP-70−/− mice in C57BL/6 background (JPG, 124 KB)
-
Wt and SWAP-70−/− mice were immunized with SRBC, or injected with PBS as control. (A) Spleen sections were stained for GC using PNA (green), B220 (red) and CD3 (blue) 6 d after immunization. (B) Percent of B follicles containing GC in spleen sections of immunized animals at d 6 after immunization. Scale bars, 200 µm. Data are from two independent experiments.
- Figure S2. Hypothetical model of the role of SWAP-70 in GC formation and PC differentiation (JPG, 64.5 KB)
-
Contrasting SWAP-70 proficient mice (A), in the absence of SWAP-70 (B), stimulated B cells may adhere in the red pulp without (2) transiting through the follicle and formation of a possible short-lived GC structure, or may rapidly travel through the follicle to form a remnant GC (1) before accumulating as early as day 6 in the red pulp. Through abnormal migration and adhesion, caused by malfunctioning integrins, these B cells receive improper signals along their routes (1) or (2), which do not support development of effective memory B cells, but cause increased generation of high-affinity Ab producing plasma cells.
|
|
