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Blood, Vol. 111, Issue 3, 1131-1137, February 1, 2008
Characterization and localization of side population (SP) cells in zebrafish kidney hematopoietic tissue
Blood Kobayashi et al.
111: 1131
Supplemental material for: Kobayashi et al
Files in this Data Supplement:
- Document S1. Materials and methods (PDF, 60.7 KB).
- Figure S1. RT-PCR analyses in COS-7 cells (PDF, 66.5 KB). -
The expressions of zAbcg2a, zAbcg2c, and gCD8 were examined by RT-PCR in the COS-7 cells that transfected with zAbcg2a (zAbcg2a / COS7), zAbcg2c (zAbcg2c / COS-7), and gCD8 (gCD8 / COS-7). The  -actin from vervet monkey (Cercopithecus aethiops) was used as an internal control. N.C. indicates negative control.
- Figure S2. Comparison of Abcg2 gene loci (PDF, 109 KB). -
The gene locations for human (H. sapiens) ABCG2, zebrafish (D. rerio) Abcg2d, and pufferfish (T. nigroviridis) Abcg2 were examined using genomic sequence database version 6 at Sanger (http://www.sanger.ac.uk/). Arrows indicate gene orientation. Dashed lines connecting boxes indicate homologous genes.
- Figure S3. Flow cytometric analyses of kidney hematopoietic cells and testicular cells (PDF, 59.1 KB). -
Cells obtained from female kidney (A), male kidney (B), and testis (C) were stained with Hoechst 33342 and analyzed by flow cytometry as described in “Materials and Methods”. The very low Hoechst fluorescence populations (arrows) are observed in male kidney and testis, but not in female kidney.
- Figure S4. CFSE-labeled SP cells in ginbuna body kidney (PDF, 62.1 KB). -
A frozen section of ginbuna body kidney is shown in green fluorescence (A) and differential interference contrast (B). (C) The merged image of (A) and (B) is shown. The CFSE-labeled SP cell (arrowheads) is localized on the surface of renal tubule. (D) A higher magnification of boxed region in (C) is shown. Dotted line shows the surface of renal tubule. All scale bars indicate 5 µm. RT shows renal tubule.
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