SEARCH:   Advanced

Blood, Vol. 111, Issue 10, 4854-4861, May 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Dynamic imaging of fibrin network formation correlated with other measures of polymerization Blood Chernysh and Weisel 111: 4854 Supplemental materials for: Chernysh and Weisel Please download the videos. Do not try to view them inside your browser. Polymerization of plasma clots was imaged in real time by using a Delta Vision Spectris Restoration microscopy system equipped with an Olympus IX–70 inverted microscope with a CCD camera. Epi-fluorescence illumination was provided by a HBO – 100 W mercury arc lamp and the excitation wavelength was selected with an electronic filterwheel containing a filter for Alexa 488, dichromatic mirror and emission filter for Alexa 488. Plasma clots were prepared by mixing pooled plasma with tissue factor and CaCl2 as described in detail in the Materials and Methods section. Examples are shown of two experiments at different magnifications. While Fig. 1 of the paper shows just a few time points during polymerization, the videos show the polymerization process in more detail over the entire time course. Files in this Data Supplement: Video 1. Taken by using an Olympus 10 × 0.45 NA U Apo objective lens (MOV, 2.86 MB) - The frame size is 336 µm × 336 µm, and time points are 8.4 sec apart, 3 frames/sec. Video 1 shows the complete sequence of events during polymerization at relatively low magnification to get an overview of the process. Video 2. Taken by using an Olympus 63 × 1.4 NA Plan Apo objective lens (MOV, 10.1 MB) - The frame size is 54.7 µm × 54.7µm, and time points are 10 sec apart, 2 frames/sec. Video 2 shows more details in terms of the behavior of individual fibers while Video 1 emphasizes more the process of network formation. In Video 2, it is possible to see that individual fibers move and that longitudinal growth continues after the gel point. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

	Blood Online is supported in part by Genentech BioOncology and Biogen Idec
	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020