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Blood, Vol. 111, Issue 11, 5359-5370, June 1, 2008
Local and systemic induction of CD4+CD25+ regulatory T-cell population by non-Hodgkin lymphoma
Blood Mittal et al.
111: 5359
Supplemental materials for: Mittal et al
Files in this Data Supplement:
- Figure S1. Absolute numbers of CD25+ Treg cells are increased in NHL patients (JPG, 20.1 KB)
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The numbers of CD25+CD127lowFoxP3 CD4+ T cells in PBMC samples from patients and healthy controls are compared. P value is calculated using rank sum test.
- Figure S2. Proportions of IL-10+ Tr1 cells within CD4+ T cell population are not significantly increased in NHL patients (JPG, 20.2 KB)
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Proportions of IL-10+ cells in the CD4+ population are compared in PBMC samples from healthy controls and patients, and involved tissue samples from patients. P values are calculated using rank sum tests.
- Figure S3. Responsiveness of PBMC from patients with localized extranodal NHL (JPG, 25.6 KB)
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Graph shows PBMC proliferation with control stimuli ConA, PPD or DPT in two patients with localized extranodal NHL (patients 19 and 20).
- Figure S4. CD25+ Treg cells from NHL involved tissue samples exhibit suppressive function (JPG, 32.4 KB)
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CD25+ T cells isolated from NHL involved tissue samples of patients (A, patient 12 and B, patient 16) were hyporesponsive to stimulation with PPD, whilst CD25− fractions proliferated strongly to the antigen. Addition of the CD25+ fraction suppressed the response of the CD25− fractions to PPD.
- Figure S5. Increased apoptosis of CD20+ tumor cells after depletion of CD25+ T cells (JPG, 45.1 KB)
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Percentages of apoptotic cells (Annexin V+ve and 7AAD−ve) with and without depletion of CD25+ T cells following culture for five days (patient 9, 10 and 18).
- Figure S6. TGF-β is not produced by NHL tumor cells (JPG, 32.5 KB)
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No detectable TGF- noted after culture of different fractions (CD25− PBMC, CD20+ tumor cells or their co-cultures) from three patients (Patients 5, 7 and 24).
- Figure S7. Anti-PDL-1 and anti-PDL-2 blocking antibodies inhibit CD25+Treg induction by tumor cells (JPG, 22.9 KB)
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CD25− PBMC from patient 12 were labeled with CFSE and co-incubated with tumor cells at a ratio of 1:1 for 5 days in the presence or absence of anti-PDL-1 or anti-PDL-2 antibodies and the proportion of CD25+FoxP3+ cells measured by flow cytometry.
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