|
|
Blood, Vol. 111, Issue 6, 3108-3115, March 15, 2008
Recruitment of circulating NK cells through decidual tissues: a possible mechanism controlling NK cell accumulation in the uterus during early pregnancy
Blood Carlino et al.
111: 3108
Supplemental materials for: Carlino et al
Files in this Data Supplement:
- Figure S6. Migration of pbNK cells from pregnant and non-pregnant women or male donors through DEC: effect of progesterone (JPG, 45.3 KB)
-
Highly purified pbNK cells isolated either from first trimester pregnant women (panel A) or male donors (panel B) or non-pregnant women (panel C) were assayed for their ability to migrate through a monolayer of primary cultures of DEC grown with or without progesterone (100 nM) using different concentrations of CXCL12/SDF-1, CX3CL1/Fractalkine or CXCL10/IP-10 as chemoattractants. All data are expressed as the mean plus SD of the percentage of migrated cells obtained from four independent experiments. *, P < 0.05, as evaluated by comparing chemoattractant versus control medium (C) induced migration using Student’s t test. No significant differences were found between migration through progesterone versus non-progesterone grown cells.
- Figure S7. Migration of CD56+CD16− pbNK cells from pregnant and non-pregnant women or male donors through DEC: effect of progesterone (JPG, 45.2 KB)
-
Highly purified pbNK cells isolated either from first trimester pregnant women (panel A) or male donors (panel B) or non-pregnant women (panel C) were assayed for their ability to migrate through a monolayer of primary cultures of DEC grown with or without progesterone (100 nM) using different concentrations of CXCL12/SDF-1, CX3CL1/Fractalkine or CXCL10/IP-10 as chemoattractants. Migrated cells were then recovered and stained with PE-conjugated anti-CD56, FITC-conjugated anti-CD16 and PERCP-conjugated anti-CD3 or control mAb and the percentage of CD56+CD16− NK cells was evaluate by cytofluorimetric analysis. Data are expressed as the ratio between the percentage of CD56highCD16negative NK cells migrated in all the tested conditions and that found before the assay. The mean plus SD of four independent experiments is shown. *, P < 0.05, as evaluated by comparing CD56highCD16negative NK cell migration index obtained for chemoattractant or control medium (C) versus that of input cells.
- Figure S8. Migration of CD56highCD16negative pbNK cells from pregnant and non-pregnant women or male donors through ST cells: effect of progesterone (JPG, 42.3 KB)
-
Highly purified pbNK cells isolated either from first trimester pregnant women (panel A) or male donors (panel B) or non-pregnant women (panel C) were assayed for their ability to migrate through a monolayer of primary cultures of ST cells grown with or without progesterone (100 nM) using different concentrations of CXCL12/SDF-1, CX3CL1/Fractalkine or CXCL10/IP-10 as chemoattractants. Migrated cells were then analyzed as described in Fig.7. The mean plus SD of four independent experiments is shown. *, P < 0.05, as evaluated by comparing the migration index of CD56+CD16− NK cells induced by chemoattractant or control medium (C) versus that of input cells.
- Figure S9. Migration of dNK cells through decidual ST cells: effect of progesterone (JPG, 29.5 KB)
-
Highly purified dNK were assayed for their ability to migrate through a monolayer of primary cultures of ST decidual cells grown with or without progesterone (100 nM).
All data are expressed as the mean plus SD of the percentage of migrated cells obtained from three independent experiments. **, P < 0.05, as evaluated by comparing migration through progesterone versus non-progesterone grown cells using Student’s t test.
- Figure S10. Chemokine receptor expression on pbNK cells co-cultured with DEC grown with or without progesterone (JPG, 69.7 KB)
-
pbNK cells from first trimester pregnant women were co-cultured with DEC grown in the presence (panel C ) or absence (panel B) of progesterone (100 nM) or with control medium (panel A) for 36 hours at 37°C. After incubation, chemokine receptor expression on gated CD56+CD3− NK cells was analyzed. Control represent staining with FITC-conjugated GAM or GARB Abs. Data are representative of two independent experiments.
|
|
