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Blood, Vol. 111, Issue 7, 3626-3634, April 1, 2008
CCL3 and CXCL12 regulate trafficking of mouse bone marrow NK cell subsets
Blood Bernardini et al.
111: 3626
Supplemental materials for: Bernardini et al
Files in this Data Supplement:
- Figure S1. Chemokine receptor cell surface expression profile on NK cell subsets from different anatomic compartments (JPG, 39.5 KB)
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(A) Cell surface expression of chemokine receptors on developing NK cells. Results presented are the mean +/− SD of the percent of positive cells of at least three independent experiments. (B, C) Expression of chemokine receptors on iNK (B) and mNK (C) cells from BM, blood and spleen. Results presented are the mean +/− SD of the percent of positive cells of six independent experiments. Student’s t test was performed by comparing cell surface expression of chemokine receptor on different BM NK cell subsets each other (A) or on each NK cell subset from different anatomic compartments (B, C) *p <0.05. Not statistically significant differences are omitted for sakes of simplicity.
- Figure S2. CXCR4 expression on BM mNK cells (JPG, 28 KB)
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BM cells were enriched for NK cells and depleted of CD3+ cells using the mouse NK cell isolation kit from Miltenyi Biotech (Auburn, CA). Cells were then stained with specific antibodies against CXCR4, NK1.1, CD11b and DX5. NK1.1+DX5+ cells were gated and CXCR4 expression was analyzed on CD11blow and CD11bhigh cells. A representative experiment out of three performed is shown on left panel and numbers in the dot plot indicate the percentage of positive cells within the CD11blow and CD11bhigh mNK cells populations. Right panel shows the mean of MFI +/− SD of CXCR4 from three experiments. Student’s t test was performed by comparing CXCR4 expression on CD11blow versus CD11bhigh mNK cells . *p <0.05.
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