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Blood, Vol. 111, Issue 7, 3435-3438, April 1, 2008
All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sac
Blood Lux et al.
111: 3435
Supplemental Materials for: Lux et al
Files in this Data Supplement:
- Table S1. Definitive HPC number (PDF, 26KB) -
A total of 84 embryos were analyzed from E8.5-9.5 (3-25sp) with a minimum of 6 embryos (# Emb) per genotype (Ncx1+/+, Ncx1+/−, Ncx1−/−) at each developmental stage. Each embryo was separated into the YS and PSp fractions, digested to single cells and plated into multiple colony forming assays depending on developmental age (# Exp). The total number of definitive HPCs per embryo (YS + PSp) was not significantly different at any stage regardless of genotype (mean ± s.e.m.). The number of definitive HPCs in the E9.0 & E9.5 Ncx1−/− PSp was significantly lower than their WT and Ncx1+/− littermates, with few or no definitive HPCs/PSp detected (*P<0.01 by paired Student t test).
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