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Blood, Vol. 111, Issue 8, 4184-4192, April 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Novel biphasic role for lymphocytes revealed during resolving inflammation Blood Rajakariar et al. 111: 4184 Supplemental materials for: Rajakariar et al Files in this Data Supplement: Figure S1. FACS analysis showing the expression of (A) the B cell surface marker, B220 along with (B) CD5 and (C) MAC-1 using the (D) IgG2a antibody as an isotype control at both onset and at resolution highlighting the increased expression of MAC-1 on B220low cells during resolution (JPG, 106 KB) - Figure S2 (JPG, 72.8 KB) - Disappearance of lymphocytes in response to inflammatory stimuli is not due to adherence to the peritoneal lining as addition of 5% trypsin for up to 10mins to an already washed out inflamed cavity at 4h did not recover either T or B cells. Panel (A) shows a typical profile of peritoneal leukocytes present at this time point comprising PMNs, monocytes and some lymphocytes. Panel (B) shows that only macrophages are recovered after trypsinising the previously washout cavity displaying a clear absence of lymphocytes as indicated. Figure S3. Profile of cells as determined by FACS at 72h in both (A) wild type animals and (B) pg91phox knockout mice (JPG, 92.4 KB) - Highlighted is the clear reduction in repopulating lymphocytes in non-resolving knockouts as well as the increased numbers of PMNs in these animals being indicative of an ongoing inflammatory response. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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