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Blood, Vol. 111, Issue 3, 1497-1503, February 1, 2008
Regulation of B-cell development by BCAP and CD19 through their binding to phosphoinositide 3-kinase
Blood Aiba et al.
111: 1497
Supplemental materials for: Aiba et al
Files in this Data Supplement:
- Figure S1. In vitro differentiation of sorted pro-B cells (JPG, 55.9 KB)
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B220+CD43+IgM− cells were sorted from bone marrow cells using FACSVantage (Becton Dickinson). The cells were cultured for 4 days in the presence of IL-7, then cultured for an additional 2 days in the absence of IL-7. The recovered cells were stained with anti-IgM and anti-IgD mAb and subjected to flow cytometric analysis.
- Figure S2. Akt activation induced by oxidative stress (JPG, 54 KB)
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Bone marrow B cells were cultured for 4 days in the presence of IL-7. The recovered cells were stimulated with 100 µM of H2O2 for the indicated periods of time. Whole-cell lysates from stimulated cells were subjected to Western blotting with anti-phospho-serine 473 Akt or anti-Akt1 antibodies.
- Figure S3. Expression of Igβ on bone marrow B cells (JPG, 52 KB)
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Bone marrow B cells were cultured for 4 days in the presence of IL-7. The recovered cells were stained with biotin-anti-Ig mAb followed by staining with streptavidin-APC. The cells were then subjected to flow cytometric analysis.
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