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Blood, Vol. 111, Issue 10, 5118-5129, May 15, 2008
Overexpressed NF- B–inducing kinase contributes to the tumorigenesis of adult T-cell leukemia and Hodgkin Reed-Sternberg cells
Blood Saitoh et al.
111: 5118
Supplemental materials for: Saitoh et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 100 KB)
- Table S1. Diagnostic information of ATL patients (JPG, 162 KB)
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- Table S2. FISH analysis of the NIK locus in PBMCs from ATL patients (JPG, 116 KB)
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- Figure S1. FISH analysis of the nik locus in PBMCs (JPG, 278 KB)
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Representative FISH images with the nik probe; RP5-1169K15 (green) and the 17q21 probe; RP11-510P20 or RP11-229K15 (red) hybridized to interphase cells from PBMCs from 3 ATL patients or 2 healthy donors. The preparations were counter-stained with DAPI. Original magnification: ×1,000.
- Figure S2. Constitutive NF-κB activity and the DNA-binding NF-κB components in MEFs transduced with NIK (JPG, 100 KB)
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MEFs were infected with retrovirus capable of expressing HA-tagged NIK (NIK) or its empty control vector (EV) and subjected to selection with puromycin (2 µg/ml). (A) Puromycin resistant cells were lysed with RIPA buffer and subjected to immunoblotting with anti-NIK, anti-p52, anti-phospho-I B , anti-IB or anti--tubulin antibodies. (B) Five micrograms of nuclear extracts prepared from retrovirus-infected MEFs were subjected to EMSA with labeled oligonucleotides encoding the NF-B-binding or Oct-1-binding sequences as probe. (C) Nuclear extracts (5 µg) prepared from retrovirus-infected MEFs were pre-incubated for 30 minutes with pre-immune (PI), anti-p50, anti-p52, anti-RelA or anti-RelB sera, and then subjected to EMSA with the NF-B-specific probe.
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