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Blood, Vol. 111, Issue 7, 3615-3625, April 1, 2008
Shedded neuronal ICAM-5 suppresses T-cell activation
Blood Tian et al.
111: 3615
Supplemental materials for: Tian et al
Files in this Data Supplement:
- Figure S1. sICAM-5 down-regulates the CD28-costimulated T cell activation (JPG, 119 KB)
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T cells were activated by immobilized anti-CD3 mAb in the absence or presence of soluble anti-CD28 mAb (0.8 µg/ml) and sICAM-5-Fc (50 µg/ml) for 18 h, and double stained for CD45RO and CD69, which are presented as dot plots (A) and percentage of CD69 expression (B). The percentage of positive cells is marked in the quadrants. In both the absence and the presence of CD28 co-stimulation, sICAM-5-Fc reduced the proportion of CD69+ T cells (A), and significantly decreased CD69 expression in activated CD45ROLow, but not in CD45ROHigh T cells (B). Data are shown as means ± SD of triplicates. *=P<0.05, **=P<0.01.
- Figure S2. Cytokine-activated bystander T cells promote the production of sICAM-5 (JPG, 61.1 KB)
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Human T cells were untreated or treated with TNF, IFN- , IL-1, or IL-8 (50 ng/ml) for 30 min at room temperature. T cells were then incubated with 14-day-old hippocampal neurons for 16 h. The conditioned media and hippocampal cell lysates were processed for Western blotting. Activation of bystander T cells by cytokines induced a dramatic release of sICAM-5 fragments into the media (A, upper right panel), with a concomitant secretion of large amounts of active MM-9 (A, lower right panel). By contrast, the above mentioned cytokines did not induce ICAM-5 cleavage directly from the treated hippocampal neurons (B).
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