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Blood, Vol. 112, Issue 5, 1720-1729, September 1, 2008
Overexpression of delta-like 4 induces arterialization and attenuates vessel formation in developing mouse embryos
Blood Trindade et al.
112: 1720
Supplemental materials for: Trindade et al
Files in this Data Supplement:
- Figure S1. Endothelial-specific overexpression of Dll4 repeats the vascular defects described in DT embryos (JPG, 191 KB)
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(A–E) Immunofluorescence with anti-PECAM1 and anti-Dll4 antibodies on WT (A), DT (B, C) and Dll4e embryos at E9.5 (D, E), confirming that Dll4 is ubiquously expressed in DT embryos and that it is pan-endothelial in Dll4e embryos, while WT embryos show only Dll4 expression on the DA. (F) Immunofluorescence with anti-PECAM1 antibody on WT embryo at E9.5. (G) Dll4e embryo at E9.5 displaying cardiac hypertrophy. (H–J) Immunofluorescence with anti-PECAM1 antibody on WT (H) and Dll4e at E9.5 (I, J), confirming that Dll4e embryos form arterio-venous malformations that are identical to the ones formed by DT embryos. (K, L) Immunofluorescence with anti-PECAM1 and anti-Fibronectin antibodies on WT (K) and Dll4e embryos (L) at E9.5, confirming that arterial endothelial cells from Dll4e embryos have a more dense basal lamina. (M, N) Immunofluorescence with anti-PECAM1 and anti-SMA antibodies on WT (M) and Dll4e embryos (N) at E9.5, revealing that DA from Dll4e embryos recruit more smooth muscle cells and so they are more stabilized. (O) BrdU incorporation studies show 6.2% of endothelial cells per aortic cross-section of E9.5 Dll4e embryo are proliferating, compared to 20.2% in WT embryos (p<0.001). (P) In E9.5 WT embryos there are on average 17.3 endothelial cells per aortic cross-section, in DT embryos there are 28.3 endothelial cells, representing a 55% increase (p<0.001).
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