SEARCH:   Advanced

Blood, Vol. 111, Issue 11, 5326-5333, June 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef IL-2 and IL-21 confer opposing differentiation programs to CD8+ T cells for adoptive immunotherapy Blood Hinrichs et al. 111: 5326 Supplemental materials for: Hinrichs et al Files in this Data Supplement: Figure S1. IL-21 acts directly on CD8+ T cells to prevent effector phenotype acquisition (JPG, 47.9 KB) - Naïve pmel-1 Thy1.1 CD8+ T cells were primed with splenocytes from Il21r−/− mice pulsed with hgp10025–33. The indicated cytokine was added at priming. (A, B, and C) Flow cytometric determination of granzyme B, CD44, and IL-2R expression by Thy1.1 lymphocytes 3 days after priming. Figure S2. IL-21 inhibits antigen-induced expression of Eomes (JPG, 21 KB) - Naïve pmel-1 Thy1.1 CD8+ T cells were primed with cognate antigen and the indicated cytokine for 3 days. Eomes expression was determined by real-time RT-PCR. Figure S3. IL-21–mediated inhibition of effector development is not due to impaired CD8+ T-cell viability (JPG, 45.5 KB) - Naïve pmel-1 Thy1.1 CD8+ T cells were primed with cognate antigen and the indicated cytokine for 3 days. Flow cytometric assessment of propidium iodide uptake by Thy1.1 lymphocytes was performed. Figure S4. IL-21–primed CD8+ T cells convert to full effector CD8+ T cells upon secondary stimulation in vivo and in vitro (JPG, 63 KB) - Pmel-1 Thy 1.1 CD8+ T cells were primed antigen-primed with 10 ng/mL of the indicated cytokine for four days. (A) 1 × 106 cells were adoptive transferred into irradiated hosts. Vaccine and IL-2 were concomitantly administered. The dot plots show expression of granzyme B and CD44 by Thy1.1 splenocytes four days after transfer. The dot plots shown are representative of four mice per group. (B) Pmel-1 CD8+ T cells were antigen-primed with the indicated cytokine for four days then restimulated in vitro with antigen and 10 ng/ml IL-2 for three days. The solid histogram indicates granzyme B expression by these cells. The open histogram reflects granzyme B expression by unstimulated control CD8+ T cells. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020