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Blood, Vol. 111, Issue 3, 1404-1412, February 1, 2008
Induction of regulatory T cell–resistant helper CD4+ T cells by bacterial vector
Blood Nishikawa et al.
111: 1404
Supplemental materials for: Nishikawa et al
Files in this Data Supplement:
- Figure S1. NY-ESO-1-specific CD4+ T cells are elicited by S. typhimurium-NY-ESO-1 from PBMC without CD4+CD25+ T cell depletion (JPG, 130 KB)
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The presence or absence of NY-ESO-1 peptide-specific CD4+ Th1 cells in whole CD4+ T cells and CD4+CD25− T cells was analyzed by proliferation assays following a 15 to 20 day culture with APC pulsed with indicated NY-ESO-1 peptides (except NC155; NY-ESO-1 157-170) or infected with S. typhimurium-NY-ESO-1 or S. typhimurium control strain (see Materials and Methods). Responses were analyzed by specific 3H incorporation for recognition of autologous T cell-depleted PBMC pulsed with an indicated peptide (except NC155; NY-ESO-1 157-170 (grey column), or HIV peptide (open column)), in 4 healthy donors and in 4 patients with NY-ESO-1 expressing tumors but without NY-ESO-1 antibody. These experiments were performed independently at least twice with similar results. Data are expressed as mean ± SD.
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