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Blood, Vol. 111, Issue 3, 1480-1488, February 1, 2008
Apoptosis of dendritic cells induced by decoy receptor 3 (DcR3)
Blood You et al.
111: 1480
Supplemental materials for: You et al
Files in this Data Supplement:
- Figure S1. Morphology of DcR3.Fc or HBD.Fc-treated macrophages (JPG, 118 KB)
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CD14+ (5 × 105) monocytes were incubated with M-CSF (10 ng/ml), in the presence of 10 µg/mL Fc-fusion proteins or IgG1, for 6 days to differentiate them into macrophages. Cells were incubated with trypan blue/PBS (0.1%) and Hoechst 33258 (1 µg/ml) for 3 minutes, and observed under phase-contrast and fluorescence microscopy, respectively.
- Figure S2 Expression of death domain-containing receptors in DCs by reverse-transcriptase (RT)-PCR after DcR3.Fc treatment for 6 hours, 18 hours, and 36 hours (JPG, 108 KB)
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IgG1 is used as control. PCR were performed for 30 cycles (lane 1, 4, 7, 10, 13, 16, 19), 25 cycles (lane 2, 5, 8, 11, 14, 17, 20), and 20 cycles (lane 3, 6, 9, 12, 15, 18, 21).
- Figure S3. CD14+ monocytes-derived dendritic cells (5 × 105) were were pretreated with PKC inhibitors for 1 hour before incubation with DcR3.Fc(10 µg/mL), HBD.Fc (10 µg/mL), or IgG1 (10 µg/mL) (JPG, 115 KB)
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Apoptotic cells were determined by annexin V-PE/7-AAD staining. *, P < 0.05 compared with control group. PKC inhibitors: Ro-31-8220 (3 µM), GF-109203X (3 µM), HBDDE (10 µM), Gö 6976 (1 µM), Gö 6983 (5 µM), LY333531 (3 µM) and LY379196 (3 µM).
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