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Blood, Vol. 111, Issue 9, 4741-4751, May 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Strong induction of 4-1BB, a growth and survival promoting costimulatory receptor, in HTLV-1–infected cultured and patients' T cells by the viral Tax oncoprotein Blood Pichler et al. 111: 4741 Supplemental materials for: Pichler et al Files in this Data Supplement: Table S1. Primers and probes used in 4-1BB and 4-1BBL mRNA detection (JPG, 46 KB) - Probes used in real-time RT-PCR were labeled with the fluorescence dye 6-carboxyfluorescein (FAM) at the 5′-end and the quencher tetramethylrhodamine (TAMRA) at the 3′-end. Figure S1. Absence of a significant calcineurin effect on 4-1BB expression in HTLV-1-transformed cells (JPG, 32.5 KB) - (A) HTLV-1-transformed cells Eva, JuanaW and StEd were cultivated for 48 hours in the presence of a calcineurin inhibitor cyclosporin A (CsA; 1g/ml), or solvent. Total cellular RNA was extracted and 4-1BB transcripts were quantified by real-time RT-PCR. Relative copy number was determined by normalizing the 4-1BB transcripts to those of -actin. (B) To analyze 4-1BB mRNA variations in time, JuanaW cells were incubated for the indicated time intervals with CsA. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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