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Blood, Vol. 111, Issue 6, 3236-3244, March 15, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure Blood Minamishima et al. 111: 3236 Supplemental materials for: Minamishima et al Files in this Data Supplement: Figure S1 (JPG, 56.4 KB) - (A) Floxed PHD2 allele frequency Flox/(Flox + Null) in different tissues in 10 week old PHD2 flox/flox;Cre-ER mice after treatment with Tamoxifen at E17.5 and at 3 weeks of age. Positions of the PCR primers are indicated by the arrows in the schematic (Closed triangle; LoxP sites, closed rectangle; exons). Genomic DNA from PHD2 +/+ and PHD2 +/− MEFs was used as a control. (B). Floxed VHL allele frequency Flox/(Flox + Null) in different tissues in 9 week old VHL flox/flox;Cre-ER mice after 2 treatments with Tamoxifen at 3 weeks of age. Positions of the PCR primers are indicated by the arrows in the schematic (Closed triangle; LoxP sites, closed rectangle; exons). Genomic DNA from VHL +/+ and VHL +/− MEFs was used as a control. Why the signals for the PHD2+/− and VHL+/− MEFs is slightly below the theoretical value of 50% is not known. Figure S2. Real-time RT-PCR analysis for PHD2 mRNA from 10 week old PHD2 +/+;Cre-ER (‘+/+’) and PHD2 flox/flox;Cre-ER (‘F/F’) mice after treatment with Tamoxifen at E17.5 and at 3 weeks of age (JPG, 44.3 KB) - Figure S3. Gross appearance (left) and H&E staining (right) for dilated inferior vena cava(IVC) of PHD2 F/F; Cre-ER 12 weeks old male, treated with Tamoxifen in-utero (dpc 17.5) and at 3 weeks of age (JPG, 139 KB) - K; kidney, Sp; spleen, Ao; aorta. Figure S4. Histological analysis (Hematoxylin and Eosin staining) of Heart from 7-week-old PHD2 F/F; Cre-ER mouse treated with tamoxifen in utero (dpc 17.5) and at 3 weeks of age (JPG, 127 KB) - Note contraction band necrosis (arrow) indicative of early ischemia. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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