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Blood, Vol. 113, Issue 5, 1045-1052, January 29, 2009
Hematopoietic cell–derived interferon controls viral replication and virus-induced disease
Blood Lang et al.
113: 1045
Supplemental materials for: Lang et al
Files in this Data Supplement:
- Figure S1. Bone marrow reconstiution of immune cells (JPG, 169 KB)
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(A–B) Bone marrow was taken form both femurs and tibiae of WT mice, irf7−∕− mice and reconstituted animals (WT-BM>WT, WT-BM>irf7−∕−, irf7−∕−-BM>WT and irf7−∕−-BM>irf7−∕−). Numbers of total bone marrow cells were analyzed by FACS (A, n=2–3). Different lineages and sca1+ ckit+ lin− stem cells were analyzed in the bone marrow (B, n=2–3). (C–D) Single cell suspensions were generated from spleens of WT mice, irf7−∕− mice and bone marrow chimeras (WT-BM>WT, WT-BM>irf7−∕−, irf7−∕−-BM>WT and irf7−∕− BM>irf7−∕−). Numbers of total red cells and leukocytes per spleen were analyzed by the FACS (C, n=2–3). Frequencies of T cells (CD3), B cells (B220) and Granolocytes (Gr1) were analyzed (D, n=2–3). (E) WT mice were irradiated with 1050rad on day −1 and on day 0 mice were reconstituted with 8 × 106 bone marrow cells from GFP transgenic mice. After 6 weeks spleens were analyzed for GFP macrophages (CD11b+Gr1−), conventional dendritic cells (CD11c+), plasmacytoid dendritic cells (PDCA-1+), granulocytes (GR-1+), B cells (B220+) and CD8+ T cells (CD8+). GFP expression of 1 out of 3 mice is shown in original histogram plot. Staining of GFP− naïve WT mice is shown as grey area.
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