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Blood, Vol. 112, Issue 1, 111-119, July 1, 2008
The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse
Blood Huang et al.
112: 111
Supplemental materials for: Huang et al
Files in this Data Supplement:
- Document 1. Supplemental materials and methods (PDF, 68.6 KB)
- Figure S1. Semi-quantitative analysis of Abl-family SH2 domain binding to phospho-HS1 (JPG, 26.3 KB)
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Jurkat T cells were treated with pervanadate for 10 minutes, and lysates were incubated with indicated amounts of soluble, recombinant GST-SH2 domains of Abl, Arg or CrkL for 2 hrs, followed by an additional 2 hrs of incubation with excess glutathione-sepharose beads. The precipitates were eluted and analyzed by immunoblotting with anti-HS1, with care to remain within the linear range of the detection system.
- Video 1. c-Abl expression is required for generation of actin-rich lamellipodia in response to TCR engagement (MOV, 4.17 MB)
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Jurkat T cells stably expressing GFP-actin were transfected with control shRNA vector (videos 1 and 2) or shAbl-3 vector (videos 3 and 4). After 48h, actin dynamics were monitored by video confocal microscopy as the cells spread on anti-TCR-coated coverslips. Images were captured every 5 seconds. A single confocal plane, just above the plane of the coverslip is shown.
- Video 2. c-Abl expression is required for generation of actin-rich lamellipodia in response to TCR engagement (MOV, 1.29 MB)
- Video 3. c-Abl expression is required for generation of actin-rich lamellipodia in response to TCR engagement (MOV, 3.59 MB)
- Video 4. c-Abl expression is required for generation of actin-rich lamellipodia in response to TCR engagement (MOV, 1.68 MB)
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