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Blood, Vol. 112, Issue 3, 568-575, August 1, 2008
Anti-CD38 antibody–mediated clearance of human repopulating cells masks the heterogeneity of leukemia-initiating cells
Blood Taussig et al.
112: 568
Supplemental materials for: Taussig et al
Files in this Data Supplement:
- Table S1. Inhibitory effect of CD38 on engraftment in three mice strains (PDF, 16.9 KB)
- Figure S1. Effect of antibody on engraftment of human cells (JPG, 28.7 KB)
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Human mononuclear cells were incubated with either test antibody or isotype control prior to washing and transplant into mice. After six weeks the percentage of human cells in the bone marrow was determined. Horizontal bars represent mean engraftment percentage. 5 million cord blood mononuclear cells were injected per mouse. There was a significant difference between control and anti-CD34 antibody (P<0.001) and anti-CD45 antibody (P<0.001).
- Figure S2. Effect of anti-CD38 antibody on engraftment at sixteen hours post transplant (JPG, 61.8 KB)
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Cord blood mononuclear cells were stained with PKH26 and then incubated with isotype control or anti-CD38 antibody (either HIT2 or HB7 clones). After washing, 1.9 million cells were transplanted via intravenous route into each mice. At sixteen hours mice were sacrificed and bone marrow underwent analysis by flow cytometry. The number of PKH26 bright cells was determined after acquisition of more than 5 × 105 events. An example is shown from control (A) and anti-CD38 antibody (B) groups. The region marks the PKH26 bright cells. (C) Both clones of anti-CD38 antibody tested reduced the number of cells in the bone marrow significantly at sixteen hours (P<0.001).
- Figure S3. Expression of CD34 and CD38 on AML samples (JPG, 129 KB)
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The expression of CD34 and CD38 on the seven AML samples that were sorted is shown. The region R1 marks the CD34+CD38+ cells and the region R2 marks the CD34+ CD38− cells. The lower left quadrant is the area in which more than 98% of unstained cells fall. The figures are the percentages of the live cells that are in each region. (A) sample 3 (B) Sample 4 (C) AML sample 6 (D) AML sample 7 (E) AML sample 8 (F) AML sample 9 (G) AML sample 10.
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