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Blood, Vol. 111, Issue 10, 5047-5053, May 15, 2008
High frequency of CD4+FoxP3+ cells in HTLV-1 infection: inverse correlation with HTLV-1–specific CTL response
Blood Toulza et al.
111: 5047
Supplemental materials for: Toulza et al
Files in this Data Supplement:
- Figure S1. Representation of the quantification of the rate of lysis for two HAM/TSP patients (JPG, 58.8 KB)
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(A) The value in each quadrant represents the percentage of cells positive for the respective protein (Tax or FoxP3), gated on the CD4+ population, in the presence of an increasing frequency of CD8+ cells, after 18hr incubation. The quadrant marked “normal” represents the physiological point, with that patient’s normal percentage of CD8+ cells. The rate of lysis value ( ) corresponding to the rate of Tax cell kill by CD8 by day. (B) Percentage of CD4 cells expressing Tax in 23 ACs and 22 HAM/TSP patients. Tax expression was assayed at the physiological ratio of CD4+:CD8+ cells (“normal”) after 18h incubation. (C) Density plot showing Tax and CD25 expression in PBMCs, gated on the CD4+ population, from a representative HAM/TSP patient (TAC) after 18h incubation.
- Figure S2. Representation of the expression of CD4 and FoxP3 expression in the Tax– population and FoxP3 expression in the Tax+ population (JPG, 16.8 KB)
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The P value was calculated by an unpaired t-test (two-tailed). Each rate was calculated by the formula indicated.
- Figure S3. Cytokine secretion detected by fluorescent microbead assay of PBMC supernatant (JPG, 57.9 KB)
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(A) Data represent spontaneous cytokine production (pg/ml) in unstimulated peripheral blood mononuclear cells (PBMCs), incubated for 18h in vitro. Samples were taken from 5 uninfected controls, 12 asymptomatic HTLV-1 carriers, and 15 patients with HAM/TSP. P values were calculated using an unpaired t-test (two-tailed). Only significant differences are shown: * P< 0·05, ** P<0·005, *** P< 0·001. (B) Representation of two typical lysis assays (two HAM/TSP patients) with a high efficiency of lysis. In these two experiments we compared the rate of lysis in the presence or absence of 2 µg/ml of IL-10.
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