|
|
Blood, Vol. 111, Issue 12, 5544-5552, June 15, 2008
The combination of granulocyte colony-stimulating factor and stem cell factor significantly increases the number of bone marrow–derived endothelial cells in brains of mice following cerebral ischemia
Blood Toth et al.
111: 5544
Supplemental materials for: Toth et al
Files in this Data Supplement:
- Figure S1 (JPG, 348 KB)
-
The section of a vessel is shown, where GFP immunopositive cells can be seen in the vascular wall (A). Y chromosome (red fluorescence) can also be seen along the vessel (B). The cell nuclei are shown in blue (DAPI). The overlay of the above colors (D) demonstrates that all GFP positive cells are also Y chromosome positive. The inset shows an enlargement of the boxed area. Objective: 20×, numerical aperture 0.7; Bar=25µm.
- Figure S2. A capillary is shown taken out of a Z series with several green GFP positive cells lining the lumen (asterisk) (JPG, 241 KB)
-
Cell nuclei are shown in blue (DAPI) (B) demonstrates two additional examples of two vessels (asterisks) that have GFP positive (green) cells at the luminal surface. Objective: 40×, numerical aperture 0.6.
- Figure S3. Section of a capillary lined with many GFP immunopositive cells (green fluorescence) indicating their bone marrow origin (JPG, 347 KB)
-
CD31 immunostaining in red fluorescence indicates the endothelial nature of the cells that are labeled. Many cells exhibit both colors and show up as yellow/orange colored. The higher magnification shows the boxed area with a few double labeled endothelial cells. Asterisks label the lumen of vessels. Traditional fluorescent microscopy using a LEICA DMI 6000 microscope. Objective: 10×, numerical aperture 0.3; Bar: 25 µm.
- Figure S4. A section (low magnification of the area around the vessel shown in Fig.5 G of the article) near the ischaemic area is shown (JPG, 489 KB)
-
Dapi staining is used to show the nuclei (A and E); GFP immunostaining shows cells that originated from the bone marrow of the donor GFP+ mice (B and F) and the red immunostaining using an antibody to BRDU indicates proliferating nuclei (C and G). D and H are overlays of the three colors. Arrows point to two GFP immunopositive endothelial cells lining the capillary wall that also have BRDU immunopositive (red) nuclei, suggesting that these cells originated from transplanted bone marrow cells and proliferated in vivo. E–H is a higher magnification of the boxed area in A–D. Asterisks indicate vascular lumen. Traditional fluorescent microscopy using a LEICA DMI 6000 microscope. Objective: 10×, numerical aperture 0.3 (A-D) and 20×, numerical aperture 0.7 (E-H). Bar: 120 µm (A-D) and 50µm (E-H).
|
|
