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Blood, Vol. 113, Issue 1, 154-164, January 1, 2009
Functional proteomic profiling of AML predicts response and survival
Blood Kornblau et al.
113: 154
Supplemental materials for: Kornblau et al
Files in this Data Supplement:
- Table S1. Proteins measured and antibodies utilized (PDF, 47.2 KB)
- Figure S1. Comparison between replicate slides stained with the same antibody (JPG, 39.8 KB)
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Slides from the same printing session were stained on separate days, approximately 2 weeks apart using the same vial of primary antibody. Absolute correlation (Pearson, R) is shown in the upper left corner and the Rank correlation (Spearman, R) is shown in the lower right corner. This demonstrates the interslide reproducibility of the RPPA technique.
- Figure S2. Protein expression in individual cases against signature groups (JPG, 94.8 KB)
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Proteins were clustered into 10 constellations shown by the 10 colored boxes along the left axis and the yellow lines surrounding each constellation. Based on the score for each constellation for each patient an overall score was generated and this divided patients into 7 protein signature groups (bottom of heatmap). This heatmap shows the expression of each protein in each individual patient, The fold change between −2 standard deviations and +2 standard deviations for each individual protein, corresponding to the −2 (green) to +2 (red) color gradient bar shown in the upper left corner, is listed to the right of the protein name.
- Figure S3. The combined effect of the simultaneous activation of multiple ERK, AKT, and PKCα was ascertained, similar to Fig. 7 in Ref. 7 (JPG, 15.3 KB)
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Each protein was divided at the median and patients divided up based on whether 0, 1,2, or all 3 were above the median. We again found that patients were more likely to have multiple pathways or no pathways activated, expected = ~ 26, (1/2)3 = 1/8*211 = 26.6) cases observed all low = 46?? And all three high = 40. Similar to our prior study we observed that prognosis was worse with STP activation, but the difference between those with 1 vs. 3 was not statistically significant.
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