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Blood, Vol. 112, Issue 7, 2869-2877, October 1, 2008 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Analysis of the linker for activation of T cells and the linker for activation of B cells in natural killer cells reveals a novel signaling cassette, dual usage in ITAM signaling, and influence on development of the Ly49 repertoire Blood Whittaker et al. 112: 2869 Supplemental materials for: Whittaker et al Files in this Data Supplement: Table S1. Unfractionated splenocytes of the indicated strains were isolated and - NK cells were identified as NK1.1+ CD3− lymphocytes. The percentage of NK cells (n=3) expressing the indicated markers are reported along with the standard deviation. Figure S1. The absolute number of splenic NK cells (NK1.1+ CD3− lymphocytes) of the indicated strains was assessed by multiplying total splenocyte numbers by the percentage of NK cells determined by flow cytometry (JPG, 44.6 KB) - p values were derived from two tailed unpaired T tests. Figure S2. ALAKs from the indicated strains were stimulated with nothing (C), plate bound anti-NK1.1, or a combination of phorbol ester and ionophore (P/I) for 6 hours (JPG, 39.1 KB) - Cells were harvested, RNA extracted and RPA analysis of mRNA for MIP1β and MIP1α was performed. Hybridization to L32 served as a loading control. Figure 3. ALAKs from the indicated strains were stimulated with nothing (C), plate bound anti-NK1.1 for 6 hours (JPG, 16.5 KB) - Cells were harvested, RNA extracted and RPA analysis of mRNA for IFNγ and IL-2 was performed. Hybridization to L32 served as a loading control. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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