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Blood, Vol. 112, Issue 2, 264-276, July 15, 2008
Coactivator function of RIP140 for NF B/RelA-dependent cytokine gene expression
Blood Zschiedrich et al.
112: 264
Supplemental materials for: Zschiedrich et al
Files in this Data Supplement:
- Table S1. Differential gene expression upon RIP140 knockout in primary macrophages (PDF, 324 KB)
- Figure S1. Real-time PCR analysis of mRNA levels for RUNX1, lysozyme (LYZS), CSF1R, and EMR1 in bone marrow-derived macrophages from wild-type (wt) or RIP140 knockout (ko) littermates (JPG, 42.3 KB)
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Data are means ± s.e.m (n ≥ 3, each done in duplicates). *, p < 0.05.
- Figure S2. Real-time PCR analysis (lower part) and Western blot analysis (upper part) of RAW264.7 macrophages infected with adenoviruses expressing 2 independent RIP140-specific or control shRNA constructs (JPG, 59.4 KB)
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60 h after infection, cells were treated with LPS (100 ng/ml) for 8 h as indicated. Proteins were detected by Western Blot using RIP140- or β-actin-specific antibody. Lane 1, shRNA control; lane 2, shRNA control plus LPS; lane 3, shRNA 1 mRIP140; lane 4, shRNA 1 mRIP140 plus LPS; lane 5, shRNA 2 mRIP140; lane 6, shRNA 2 mRIP140 plus LPS.
- Figure S3. Real-time PCR analysis of mRNA levels for IL1β, IL6, and TNFα in RAW264.7 macrophages infected with adenoviruses expressing 2 independent RIP140-specific or control shRNA constructs (JPG, 50.4 KB)
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60 h after infection, RAW264.7 cells were treated with LPS (100 ng/ml) for 8 h as indicated. Data are means ± s.e.m (n=9). *, p < 0.05.
- Figure S4. Western blot analysis of RAW264.7 macrophages infected with an adenovirus expressing a RIP140-specific or control shRNA construct (JPG, 34.6 KB)
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60 h after infection, cells were treated with LPS (100 ng/ml) for 8 h or left untreated. Proteins were detected by Western Blot using RIP140-, RelA, or β-actin-specific antibody. Lane 1, shRNA control; lane 2, shRNA control plus LPS; lane 3, shRNA 1 mRIP140; lane 4, shRNA 1 mRIP140 plus LPS.
- Figure S5. Transient transfection assay of RAW264.7 macrophages co-transfected with pκB-Luc (containing wild-type NFκB binding sites) together with RelA and 2 independent RIP140-specific or non-specific control shRNA constructs as indicated (JPG, 44.3 KB)
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Data are means ± s.e.m (n=9). *, p < 0.05.
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